Progress of the Study on the Genetic Resources of Avocado Iv. Use of the Spacer Size Variation of Ribosomal 5s Genes to Identify the Horticultural Race of Avocado Genotypes
نویسنده
چکیده
'Universal' primers have been used to amplify the nontranscribed spacer (NTS) of the ribosomal 5S gene of avocado genotypes by the polymerase chain reaction (PCR). A rich size heterogeneity of NTSs of individual avocado genotypes was detected after high resolution polyacrylamide gel electrophoresis and silver staining. Specific sizes of NTS seem to be common for members of each horticultural race of avocado and may be used as a diagnostic tool to identify the horticultural race of avocado genotypes.
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